CRISPR Livestock and Poultry Knockout Libraries

Cellecta has designed and produced CRISPR Chicken and Pig Genome-Wide Knockout Libraries to enable convenient, genome-wide dropout viability screens. CRISPR screening of livestock and poultry could be useful in the study of pathogen resistance, improved breeding, and more.

Chicken

With around 69,000 constructs, the CRISPR chicken (Gallus gallus) library targets all chicken protein-coding genes (~17,000) with approximately four sgRNAs/gene.

• Genome-Wide Coverage with a Single 69K Module, with ~69,000 constructs
• Each gene is covered by around four sgRNAs
• 1000 Non-Targeting Controls and 100 Intron-Targeting Controls are also included
• All chicken protein-coding genes are covered—approximately 17,000 gene targets

Pig

Cellecta's CRISPR Pig (Sus scrofa) Genome-Wide Knockout Library contains around 89,000 constructs, and targets all pig protein-coding genes (~22,000) with approximately four sgRNAs/gene.

• Genome-Wide Coverage with a Single 89K Module–a single library with ~89,000 constructs
• Each gene is covered by around four sgRNAs
• 1000 Non-Targeting Controls and 100 Intron-Targeting Controls are also included
• All pig protein-coding genes covered—approximately 22,000 gene targets

In addition to providing a variety of products useful for CRISPR screening, we also run CRISPR screens as a service.

Manual and Product Documentation

• Manual: CRISPR Pooled Lentiviral sgRNA Libraries
• Gene List:
  • Chicken
  • Pig

Library Vector Information

* Sequences are available in FASTA (.txt), GenBank (.gb), and SnapGene (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us. To download .txt and .gb files, right click on the link and Save As. 

Search Product Certificates by Lot# or Catalog#

Alignment Software Supporting Files

• Library Configuration Files (JSON format, compressed):
  • Chicken
  • Pig
• Sample Description Input Form:XLSM (Please enable macros)